Nucleic acids may be labeled at their 3’ end, 5’ end, or throughout the molecule, depending on the desired application. Myriad of enzymatic or chemical methods are utilized to generate nucleic acids ...
A plethora of chemical or enzymatic methods are available to generate nucleic acids such as those labeled with fluorophores, enzymes and radioactive phosphates, or nucleotides modified with ...
We tested the following fluorescent dyes for their efficacy in the custom labeling reaction: amino-methyl coumarin (AMCA); diethyl aminomethyl coumarin (DEAC); Cascade Blue; fluorescein isothiocyanate ...
The ability to label nucleic acids with a variety of tagged molecules has led to astonishing advances in molecular biology. Labeled DNA, RNA, and oligonucleotide probes have made possible techniques ...
About nonradioactive nucleic acid detection -- Isolation of DNA from plants -- Isolation of high-molecular-weight DNA from animal cells -- Restriction enzyme digestion, gel electrophoresis and vacuum ...
The metanephric kidney was studied in fetal and older mice beginning at 16 days after mating of the parents. Polyribosomes from fetal kidneys labeled in vitro with 14C-labeled amino acids had 10-20 ...
Nucleic acids are complex biological molecules that store and transmit genetic information in all living organisms. They are essential for the survival and reproduction of life on Earth. The two main ...